TY - JOUR
T1 - In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae)
AU - Surco-Laos, Felipe
AU - Garcia, Jorge A.
AU - Bendezú, María R.
AU - Laos-Anchante, Doris
AU - Panay-Centeno, Juan F.
AU - Valle-Campos, Manuel
AU - Palomino-Jhong, Juan J.
AU - Yarasca-Carlos, Paulina Eliades
AU - Loja, Berta
AU - Alvarado, Angel T.
N1 - Publisher Copyright:
© 2022 Journal of Pharmacy & Pharmacognosy Research.
PY - 2022/11
Y1 - 2022/11
N2 - Context: Senecio nutans Sch. Beep. is used by the Andean population of Ayacucho-Peru as traditional medicine for various health problems. Aims: To evaluate the antioxidant and antimicrobial activity of the ethanolic extract of leaves of S. nutans against the enteropathogen Escherichia coli and the filamentous fungus Aspergillus niger. Methods: The antioxidant capacity of the ethanolic extract was evaluated by ABTS, DPPH, and FRAP assays; the antimicrobial activity was evaluated by the agar diffusion method, and determining the minimum inhibitory concentration (MIC), minimum bactericidal (MBC), and fungicidal concentration (MFC). Results: Polyphenols, terpenes, and tannins were identified; the total polyphenolic content was 67.91 ± 0.29 mg GAE/g. The ethanolic extract at 20 mg/mL showed moderate antibacterial activity (79.14 ± 0.02% inhibition, MIC and MBC >400 μg/mL on E. coli), and antifungal (88.90 ± 0.17% inhibition, MIC and MCF >400 μg/mL on A. niger); radical scavenging capacity (ABTS 10.31 ± 0.09 mM TE/g; DPPH 8.28 ± 0.07 IC50 µg/mL) and iron reducing power (FRAP 17.72 ± 0.11 mM TE/g). S. nutans turned out to be a potential antimicrobial and antioxidant species associated with the presence of its bioactive components, for which further investigation is warranted. Conclusions: The ethanolic extract of leaves S. nutans showed moderate activity against pathogenic bacteria (E. coli) and filamentous fungus (A. niger), as well as antioxidant activity in three in vitro methods.
AB - Context: Senecio nutans Sch. Beep. is used by the Andean population of Ayacucho-Peru as traditional medicine for various health problems. Aims: To evaluate the antioxidant and antimicrobial activity of the ethanolic extract of leaves of S. nutans against the enteropathogen Escherichia coli and the filamentous fungus Aspergillus niger. Methods: The antioxidant capacity of the ethanolic extract was evaluated by ABTS, DPPH, and FRAP assays; the antimicrobial activity was evaluated by the agar diffusion method, and determining the minimum inhibitory concentration (MIC), minimum bactericidal (MBC), and fungicidal concentration (MFC). Results: Polyphenols, terpenes, and tannins were identified; the total polyphenolic content was 67.91 ± 0.29 mg GAE/g. The ethanolic extract at 20 mg/mL showed moderate antibacterial activity (79.14 ± 0.02% inhibition, MIC and MBC >400 μg/mL on E. coli), and antifungal (88.90 ± 0.17% inhibition, MIC and MCF >400 μg/mL on A. niger); radical scavenging capacity (ABTS 10.31 ± 0.09 mM TE/g; DPPH 8.28 ± 0.07 IC50 µg/mL) and iron reducing power (FRAP 17.72 ± 0.11 mM TE/g). S. nutans turned out to be a potential antimicrobial and antioxidant species associated with the presence of its bioactive components, for which further investigation is warranted. Conclusions: The ethanolic extract of leaves S. nutans showed moderate activity against pathogenic bacteria (E. coli) and filamentous fungus (A. niger), as well as antioxidant activity in three in vitro methods.
KW - Senecio nutans
KW - antimicrobial activity
KW - chachacoma
KW - in vitro antioxidant activity
KW - phenolic compounds
UR - http://www.scopus.com/inward/record.url?scp=85141953378&partnerID=8YFLogxK
U2 - 10.56499/jppres22.1471_10.6.1026
DO - 10.56499/jppres22.1471_10.6.1026
M3 - Artículo
AN - SCOPUS:85141953378
SN - 0719-4250
VL - 10
SP - 1026
EP - 1036
JO - Journal of Pharmacy and Pharmacognosy Research
JF - Journal of Pharmacy and Pharmacognosy Research
IS - 6
ER -