Caracterización in silico y análisis de la expresión de la subunidad alfa de la Acetil-Coenzima a carboxilasa heteromérica de dos microalgas

Juan C. Castro, J. Dylan Maddox, Segundo L. Estela, Hicler N. Rodríguez, María Z. Casuso, Jae D. Paredes, Marianela Cobos

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Microalgae are photosynthetic microorganisms with great potential to supply the world’s energy demands. However, the limited knowledge of these organisms, particularly at the molecular level of metabolic processes, has limited their use to these purposes. In this investigation, the in silico analysis of the alpha subunit of the heteromeric acetyl-coenzyme A carboxylase (αACCase), a key enzyme in lipid biosynthesis of microalgae Chlorella sp. and Scenedesmus sp. was carried out. Also, the expression of this gene has been measured in both species cultivated in nitrogen-depleted media. Results indicate that ACCase shows structural and functional conservation in both species of microalgae and their greater genetic similarity with other species of microalgae. Also, it has been shown that the expression levels of this gene are significantly increased when the microalgae are cultured in the absence of nitrogen, which in turn is related to a greater accumulation of microalgal lipids. In conclusion, the in silico analysis of the Chlorella sp. and Scenedesmus sp. αACCase reveals structural, functional and evolutionary characteristics very similar to other microalgae and plant species. Also, the study reveals that in both species the gene is overexpressed when microalgae are subjected to nitrogen deficiency stress, which is significantly related to total lipids accumulation in these cells.

Título traducido de la contribuciónIn silico characterization and expression analysis of the alpha subunit of the heteromeric acetyl-coenzyme a carboxylase from two microalgae
Idioma originalEspañol
Páginas (desde-hasta)275-290
Número de páginas16
PublicaciónActa Biologica Colombiana
Volumen24
N.º2
DOI
EstadoPublicada - 2019
Publicado de forma externa

Nota bibliográfica

Publisher Copyright:
© 2019, Universidad Nacional de Colombia. All right reserved.

Palabras clave

  • 3D molecular structure
  • Biological stress
  • Computational biology
  • Genetic transcription
  • Lipogenesis

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