Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride

Mayte Del C. Nerey; Sergio E. Pichuantes; Claudia P. Saavedra; Manuel A. Araya; Juan C. Tantaleán; Claudio C. Vásquez

doi:10.1007/s00284-001-0114-5

Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride

Mayte Del C. Nerey, Sergio E. Pichuantes, Claudia P. Saavedra, Manuel A. Araya, Juan C. Tantaleán, Claudio C. Vásquez

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5 Citas (Scopus)

Resumen

Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cada or cadC/cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.

Idioma original	Inglés
Páginas (desde-hasta)	187-190
Número de páginas	4
Publicación	Current Microbiology
Volumen	45
N.º	3
DOI	https://doi.org/10.1007/s00284-001-0114-5
Estado	Publicada - 1 set. 2002
Publicado de forma externa	Sí

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title = "Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride",

abstract = "Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cada or cadC/cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.",

author = "Nerey, {Mayte Del C.} and Pichuantes, {Sergio E.} and Saavedra, {Claudia P.} and Araya, {Manuel A.} and Tantale{\'a}n, {Juan C.} and V{\'a}squez, {Claudio C.}",

year = "2002",

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doi = "10.1007/s00284-001-0114-5",

language = "Ingl{\'e}s",

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TY - JOUR

T1 - Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride

AU - Nerey, Mayte Del C.

AU - Pichuantes, Sergio E.

AU - Saavedra, Claudia P.

AU - Araya, Manuel A.

AU - Tantaleán, Juan C.

AU - Vásquez, Claudio C.

PY - 2002/9/1

Y1 - 2002/9/1

N2 - Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cada or cadC/cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.

AB - Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cada or cadC/cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.

UR - http://www.scopus.com/inward/record.url?scp=0036717009&partnerID=8YFLogxK

U2 - 10.1007/s00284-001-0114-5

DO - 10.1007/s00284-001-0114-5

M3 - Artículo

C2 - 12177740

AN - SCOPUS:0036717009

SN - 0343-8651

VL - 45

SP - 187

EP - 190

JO - Current Microbiology

JF - Current Microbiology

IS - 3

ER -

Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride

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