TY - JOUR
T1 - Anticonvulsant effect of ethanolic extract of Cyperus articulatus L. leaves on pentylenetetrazol induced seizure in mice
AU - Herrera-Calderon, Oscar
AU - Santiváñez-Acosta, Rocio
AU - Pari-Olarte, Bertha
AU - Enciso-Roca, Edwin
AU - Campos Montes, Vicente Martin
AU - Luis Arroyo Acevedo, Jorge
N1 - Publisher Copyright:
© 2017 Center for Food and Biomolecules, National Taiwan University
PY - 2018/1
Y1 - 2018/1
N2 - Cyperus articulatus (CA) rhizomes have demonstrated different properties on nervous system. However, the leaves still have not studied to treat epilepsy. The aim of this study was to determine the effect of CA ethanolic extract on pentylenetetrazol (PTZ) induced seizures in mice as well as measuring its antioxidant activity in vivo and in vitro. Mice were divided into five groups: (1) control (PTZ 80 mg/kg; i.p.), (2) PTZ-Diazepam (1 mg/kg; i.p.), (3–5) PTZ-CA 50, PTZ-CA 150 and PTZ-CA 300 (50, 150 and 300 mg/kg of CA extract, 30 min prior to each PTZ injection). The PTZ-CA 150 group showed lower seizure scores (P < 0.01), latency (P < 0.01), frequency (P < 0.01) and duration (P < 0.01) than control group. The antioxidant activity of CA extract scavenged DPPH radical showed IC 50 = 16.9 ± 0.1 μg/mL and TEAC = 2.28 ± 0.08, mmol trolox/g of extract, the content of gamma amino butyric acid (GABA) and malondialdehyde (MDA) were significantly high (P < 0.01) at dose of 150 mg/kg (82 ± 1.2 ng/g tissue; 1.0 ± 2.2 mol/g tissue, respectively). The present research demonstrated that CA extract possesses a potential effect to prevent PTZ induced seizures, antioxidant activity in addition to increase GABA levels.
AB - Cyperus articulatus (CA) rhizomes have demonstrated different properties on nervous system. However, the leaves still have not studied to treat epilepsy. The aim of this study was to determine the effect of CA ethanolic extract on pentylenetetrazol (PTZ) induced seizures in mice as well as measuring its antioxidant activity in vivo and in vitro. Mice were divided into five groups: (1) control (PTZ 80 mg/kg; i.p.), (2) PTZ-Diazepam (1 mg/kg; i.p.), (3–5) PTZ-CA 50, PTZ-CA 150 and PTZ-CA 300 (50, 150 and 300 mg/kg of CA extract, 30 min prior to each PTZ injection). The PTZ-CA 150 group showed lower seizure scores (P < 0.01), latency (P < 0.01), frequency (P < 0.01) and duration (P < 0.01) than control group. The antioxidant activity of CA extract scavenged DPPH radical showed IC 50 = 16.9 ± 0.1 μg/mL and TEAC = 2.28 ± 0.08, mmol trolox/g of extract, the content of gamma amino butyric acid (GABA) and malondialdehyde (MDA) were significantly high (P < 0.01) at dose of 150 mg/kg (82 ± 1.2 ng/g tissue; 1.0 ± 2.2 mol/g tissue, respectively). The present research demonstrated that CA extract possesses a potential effect to prevent PTZ induced seizures, antioxidant activity in addition to increase GABA levels.
KW - Anticonvulsant
KW - Antioxidant
KW - Cyperus articulatus
KW - Pentylenetetrazol
UR - http://www.scopus.com/inward/record.url?scp=85018597662&partnerID=8YFLogxK
U2 - 10.1016/j.jtcme.2017.03.001
DO - 10.1016/j.jtcme.2017.03.001
M3 - Artículo
AN - SCOPUS:85018597662
SN - 2225-4110
VL - 8
SP - 95
EP - 99
JO - Journal of Traditional and Complementary Medicine
JF - Journal of Traditional and Complementary Medicine
IS - 1
ER -