The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria

Derie E. Fuentes; Claudio A. Navarro; Juan C. Tantaleán; Manuel A. Araya; Claudia P. Saavedra; José M. Pérez; Iván L. Calderón; Phil A. Youderian; Guido C. Mora; Claudio C. Vásquez

doi:10.1016/j.resmic.2005.01.002

The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria

Derie E. Fuentes
, Claudio A. Navarro
, Juan C. Tantaleán
, Manuel A. Araya
, Claudia P. Saavedra
, José M. Pérez
, Iván L. Calderón
, Phil A. Youderian
, Guido C. Mora
, Claudio C. Vásquez

Lab. de Microbiol. Molecular
Universidad de Santiago de Chile
Depto. de Ciencias Biológicas
Universidad Andrés Bello
Department of Biology
Texas A&M University

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We have characterized a natural isolate of Staphylococcus epidermidis resistant to heavy metals that carries a small 2391-bp plasmid, pSepCH, encoding the qacC gene. The S. epidermidis qacC gene confers resistance to a number of β-lactam antibiotics and to ethidium bromide in its natural host and in Escherichia coli K12 and Salmonella enterica sv. Typhimurium. This is the first communication of a small multidrug resistance (SMR) pump involved in resistance to β-lactam antibiotics. Experiments using tolC, ompW and ompD mutant strains of S. Typhimurium demonstrated that the β-lactam antibiotic resistance conferred by this pump does not depend on these outer membrane proteins.

Original language	English
Pages (from-to)	472-477
Number of pages	6
Journal	Research in Microbiology
Volume	156
Issue number	4
DOIs	https://doi.org/10.1016/j.resmic.2005.01.002
State	Published - May 2005

Keywords

Ethidium bromide
Multidrug resistance
SMR
Staphylococcus epidermidis
qacC
β-Lactam resistance

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10.1016/j.resmic.2005.01.002

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Fuentes, D. E., Navarro, C. A., Tantaleán, J. C., Araya, M. A., Saavedra, C. P., Pérez, J. M., Calderón, I. L., Youderian, P. A., Mora, G. C., & Vásquez, C. C. (2005). The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria. Research in Microbiology, 156(4), 472-477. https://doi.org/10.1016/j.resmic.2005.01.002

@article{218c79a14bd44e0eb9028df4a85f167a,

title = "The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria",

abstract = "We have characterized a natural isolate of Staphylococcus epidermidis resistant to heavy metals that carries a small 2391-bp plasmid, pSepCH, encoding the qacC gene. The S. epidermidis qacC gene confers resistance to a number of β-lactam antibiotics and to ethidium bromide in its natural host and in Escherichia coli K12 and Salmonella enterica sv. Typhimurium. This is the first communication of a small multidrug resistance (SMR) pump involved in resistance to β-lactam antibiotics. Experiments using tolC, ompW and ompD mutant strains of S. Typhimurium demonstrated that the β-lactam antibiotic resistance conferred by this pump does not depend on these outer membrane proteins.",

keywords = "Ethidium bromide, Multidrug resistance, SMR, Staphylococcus epidermidis, qacC, β-Lactam resistance",

author = "Fuentes, \{Derie E.\} and Navarro, \{Claudio A.\} and Tantale{\'a}n, \{Juan C.\} and Araya, \{Manuel A.\} and Saavedra, \{Claudia P.\} and P{\'e}rez, \{Jos{\'e} M.\} and Calder{\'o}n, \{Iv{\'a}n L.\} and Youderian, \{Phil A.\} and Mora, \{Guido C.\} and V{\'a}squez, \{Claudio C.\}",

year = "2005",

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doi = "10.1016/j.resmic.2005.01.002",

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volume = "156",

pages = "472--477",

journal = "Research in Microbiology",

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Fuentes, DE, Navarro, CA, Tantaleán, JC, Araya, MA, Saavedra, CP, Pérez, JM, Calderón, IL, Youderian, PA, Mora, GC & Vásquez, CC 2005, 'The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria', Research in Microbiology, vol. 156, no. 4, pp. 472-477. https://doi.org/10.1016/j.resmic.2005.01.002

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T1 - The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria

AU - Fuentes, Derie E.

AU - Navarro, Claudio A.

AU - Tantaleán, Juan C.

AU - Araya, Manuel A.

AU - Saavedra, Claudia P.

AU - Pérez, José M.

AU - Calderón, Iván L.

AU - Youderian, Phil A.

AU - Mora, Guido C.

AU - Vásquez, Claudio C.

PY - 2005/5

Y1 - 2005/5

N2 - We have characterized a natural isolate of Staphylococcus epidermidis resistant to heavy metals that carries a small 2391-bp plasmid, pSepCH, encoding the qacC gene. The S. epidermidis qacC gene confers resistance to a number of β-lactam antibiotics and to ethidium bromide in its natural host and in Escherichia coli K12 and Salmonella enterica sv. Typhimurium. This is the first communication of a small multidrug resistance (SMR) pump involved in resistance to β-lactam antibiotics. Experiments using tolC, ompW and ompD mutant strains of S. Typhimurium demonstrated that the β-lactam antibiotic resistance conferred by this pump does not depend on these outer membrane proteins.

AB - We have characterized a natural isolate of Staphylococcus epidermidis resistant to heavy metals that carries a small 2391-bp plasmid, pSepCH, encoding the qacC gene. The S. epidermidis qacC gene confers resistance to a number of β-lactam antibiotics and to ethidium bromide in its natural host and in Escherichia coli K12 and Salmonella enterica sv. Typhimurium. This is the first communication of a small multidrug resistance (SMR) pump involved in resistance to β-lactam antibiotics. Experiments using tolC, ompW and ompD mutant strains of S. Typhimurium demonstrated that the β-lactam antibiotic resistance conferred by this pump does not depend on these outer membrane proteins.

KW - Ethidium bromide

KW - Multidrug resistance

KW - SMR

KW - Staphylococcus epidermidis

KW - qacC

KW - β-Lactam resistance

UR - https://www.scopus.com/pages/publications/20944442633

U2 - 10.1016/j.resmic.2005.01.002

DO - 10.1016/j.resmic.2005.01.002

M3 - Artículo

C2 - 15862444

AN - SCOPUS:20944442633

SN - 0923-2508

VL - 156

SP - 472

EP - 477

JO - Research in Microbiology

JF - Research in Microbiology

IS - 4

ER -

The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria

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Keywords

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