The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria

Derie E. Fuentes; Claudio A. Navarro; Juan C. Tantaleán; Manuel A. Araya; Claudia P. Saavedra; José M. Pérez; Iván L. Calderón; Phil A. Youderian; Guido C. Mora; Claudio C. Vásquez

doi:10.1016/j.resmic.2005.01.002

The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria

Derie E. Fuentes, Claudio A. Navarro, Juan C. Tantaleán, Manuel A. Araya, Claudia P. Saavedra, José M. Pérez, Iván L. Calderón, Phil A. Youderian, Guido C. Mora, Claudio C. Vásquez

CIENCIAS BIOLÓGICAS

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We have characterized a natural isolate of Staphylococcus epidermidis resistant to heavy metals that carries a small 2391-bp plasmid, pSepCH, encoding the qacC gene. The S. epidermidis qacC gene confers resistance to a number of β-lactam antibiotics and to ethidium bromide in its natural host and in Escherichia coli K12 and Salmonella enterica sv. Typhimurium. This is the first communication of a small multidrug resistance (SMR) pump involved in resistance to β-lactam antibiotics. Experiments using tolC, ompW and ompD mutant strains of S. Typhimurium demonstrated that the β-lactam antibiotic resistance conferred by this pump does not depend on these outer membrane proteins.

Original language	English
Pages (from-to)	472-477
Number of pages	6
Journal	Research in Microbiology
Volume	156
Issue number	4
DOIs	https://doi.org/10.1016/j.resmic.2005.01.002
State	Published - May 2005

Bibliographical note

Funding Information:
The authors thank Le Phung and Simon Silver (University of Chicago) for helping with 16S rDNA sequencing. D.E.F. and J.M.P. received fellowships from MECESUP and C.A.N. and I.L.C. received fellowships from Conicyt. This work was supported by FONDECYT grants 1030234 to C.C.V. and 1020485 to G.C.M., and by NIH Fogarty Center Senior International Fellowship TW05645 to P.Y.

Keywords

Ethidium bromide
Multidrug resistance
SMR
Staphylococcus epidermidis
qacC
β-Lactam resistance

Access to Document

10.1016/j.resmic.2005.01.002

Cite this

Fuentes, D. E., Navarro, C. A., Tantaleán, J. C., Araya, M. A., Saavedra, C. P., Pérez, J. M., Calderón, I. L., Youderian, P. A., Mora, G. C., & Vásquez, C. C. (2005). The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria. Research in Microbiology, 156(4), 472-477. https://doi.org/10.1016/j.resmic.2005.01.002

@article{c45549f43f70478c97c2eac565632a6a,

title = "The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria",

abstract = "We have characterized a natural isolate of Staphylococcus epidermidis resistant to heavy metals that carries a small 2391-bp plasmid, pSepCH, encoding the qacC gene. The S. epidermidis qacC gene confers resistance to a number of β-lactam antibiotics and to ethidium bromide in its natural host and in Escherichia coli K12 and Salmonella enterica sv. Typhimurium. This is the first communication of a small multidrug resistance (SMR) pump involved in resistance to β-lactam antibiotics. Experiments using tolC, ompW and ompD mutant strains of S. Typhimurium demonstrated that the β-lactam antibiotic resistance conferred by this pump does not depend on these outer membrane proteins.",

keywords = "Ethidium bromide, Multidrug resistance, SMR, Staphylococcus epidermidis, qacC, β-Lactam resistance",

author = "Fuentes, {Derie E.} and Navarro, {Claudio A.} and Tantale{\'a}n, {Juan C.} and Araya, {Manuel A.} and Saavedra, {Claudia P.} and P{\'e}rez, {Jos{\'e} M.} and Calder{\'o}n, {Iv{\'a}n L.} and Youderian, {Phil A.} and Mora, {Guido C.} and V{\'a}squez, {Claudio C.}",

note = "Funding Information: The authors thank Le Phung and Simon Silver (University of Chicago) for helping with 16S rDNA sequencing. D.E.F. and J.M.P. received fellowships from MECESUP and C.A.N. and I.L.C. received fellowships from Conicyt. This work was supported by FONDECYT grants 1030234 to C.C.V. and 1020485 to G.C.M., and by NIH Fogarty Center Senior International Fellowship TW05645 to P.Y.",

year = "2005",

month = may,

doi = "10.1016/j.resmic.2005.01.002",

language = "Ingl{\'e}s",

volume = "156",

pages = "472--477",

journal = "Research in Microbiology",

issn = "0923-2508",

publisher = "Elsevier Masson SAS",

number = "4",

}

Fuentes, DE, Navarro, CA, Tantaleán, JC, Araya, MA, Saavedra, CP, Pérez, JM, Calderón, IL, Youderian, PA, Mora, GC & Vásquez, CC 2005, 'The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria', Research in Microbiology, vol. 156, no. 4, pp. 472-477. https://doi.org/10.1016/j.resmic.2005.01.002

TY - JOUR

T1 - The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria

AU - Fuentes, Derie E.

AU - Navarro, Claudio A.

AU - Tantaleán, Juan C.

AU - Araya, Manuel A.

AU - Saavedra, Claudia P.

AU - Pérez, José M.

AU - Calderón, Iván L.

AU - Youderian, Phil A.

AU - Mora, Guido C.

AU - Vásquez, Claudio C.

N1 - Funding Information: The authors thank Le Phung and Simon Silver (University of Chicago) for helping with 16S rDNA sequencing. D.E.F. and J.M.P. received fellowships from MECESUP and C.A.N. and I.L.C. received fellowships from Conicyt. This work was supported by FONDECYT grants 1030234 to C.C.V. and 1020485 to G.C.M., and by NIH Fogarty Center Senior International Fellowship TW05645 to P.Y.

PY - 2005/5

Y1 - 2005/5

N2 - We have characterized a natural isolate of Staphylococcus epidermidis resistant to heavy metals that carries a small 2391-bp plasmid, pSepCH, encoding the qacC gene. The S. epidermidis qacC gene confers resistance to a number of β-lactam antibiotics and to ethidium bromide in its natural host and in Escherichia coli K12 and Salmonella enterica sv. Typhimurium. This is the first communication of a small multidrug resistance (SMR) pump involved in resistance to β-lactam antibiotics. Experiments using tolC, ompW and ompD mutant strains of S. Typhimurium demonstrated that the β-lactam antibiotic resistance conferred by this pump does not depend on these outer membrane proteins.

AB - We have characterized a natural isolate of Staphylococcus epidermidis resistant to heavy metals that carries a small 2391-bp plasmid, pSepCH, encoding the qacC gene. The S. epidermidis qacC gene confers resistance to a number of β-lactam antibiotics and to ethidium bromide in its natural host and in Escherichia coli K12 and Salmonella enterica sv. Typhimurium. This is the first communication of a small multidrug resistance (SMR) pump involved in resistance to β-lactam antibiotics. Experiments using tolC, ompW and ompD mutant strains of S. Typhimurium demonstrated that the β-lactam antibiotic resistance conferred by this pump does not depend on these outer membrane proteins.

KW - Ethidium bromide

KW - Multidrug resistance

KW - SMR

KW - Staphylococcus epidermidis

KW - qacC

KW - β-Lactam resistance

UR - http://www.scopus.com/inward/record.url?scp=20944442633&partnerID=8YFLogxK

U2 - 10.1016/j.resmic.2005.01.002

DO - 10.1016/j.resmic.2005.01.002

M3 - Artículo

C2 - 15862444

AN - SCOPUS:20944442633

SN - 0923-2508

VL - 156

SP - 472

EP - 477

JO - Research in Microbiology

JF - Research in Microbiology

IS - 4

ER -

The product of the qacC gene of Staphylococcus epidermidis CH mediates resistance to β-lactam antibiotics in Gram-positive and Gram-negative bacteria

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this