Metagenomic 16S rDNA amplicon data on bacterial diversity profiling and its predicted metabolic functions of varillales in Allpahuayo-Mishana National Reserve

Juan C. Castro, J. Dylan Maddox, Hicler N. Rodríguez, Richard B. Orbe, Gad E. Grandez, Kevin A. Feldheim, Marianela Cobos, Jae D. Paredes, Carlos G. Castro, Jorge L. Marapara, Pedro M. Adrianzén, Janeth Braga

Research output: Contribution to journalArticlepeer-review

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The white-sands forests or varillales of the Peruvian Amazon are characterized by their distinct physical characteristics, patchy distribution, and endemism [1, 2]. Much research has been conducted on the specialized plant and animal communities that inhabit these ecosystems, yet their soil microbiomes have yet to be studied. Here we provide metagenomic 16S rDNA amplicon data of soil microbiomes from three types of varillales in Allpahuayo-Mishana National Reserve near Iquitos, Peru. Composite soil samples were collected from very low varillal, high-dry varillal, and high-wet varillal. Purified metagenomic DNA was used to prepare and sequence 16S rDNA metagenomic libraries on the Illumina MiqSeq platform. Raw paired-endsequences were analyzed using the Metagenomics RAST server (MG-RAST) and Parallel-Meta3 software and revealed the existence of a high percentage of undiscovered sequences, potentially indicating specialized bacterial communities in these forests. Also, were predicted several metabolic functions in this dataset. The raw sequence data in fastq format is available in the public repository Discover Mendeley Data ( Also, is available at NCBI's Sequence Read Archive (SRA) with accession numbers SRX7891206 (very low varillal), SRX7891207 (high-dry varillal), and SRX7891208 (high-wet varillal).

Original languageEnglish
Article number105625
JournalData in Brief
StatePublished - Jun 2020
Externally publishedYes

Bibliographical note

Funding Information:
Authors received financial support from the Universidad Nacional de la Amazonia Peruana through the grants “Isolation of Environmental DNA from Soil and Water Samples of the Loreto Region” (grant ID: RR N° 0189-2018-UNAP) and “Bioprospection of Genes with Biotechnological Potential in Metagenomes of the Loreto Region” (grant ID: RR N° 0421-2019-UNAP). Additionally, we thank the Laboratorio de Biotecnología y Bioenergética, Universidad Científica del Perú (UCP) and the Pritzker Laboratory for Molecular Systematics and Evolution, Field Museum of Natural History for providing necessary infrastructure and equipment. Finally, we thank the Servicio Nacional de Áreas Naturales Protegidas por el Estado (SERNANP) and the High Direction of the Reserva Natural Allpahuayo-Mishana for providing the authorization to collect the soil samples.

Publisher Copyright:
© 2020 The Author(s)


  • 16S rRNA
  • Metagenomics
  • Peruvian amazon
  • Soil microbiome
  • Tropical forest
  • Varillales
  • White-sand forests


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