Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride

Mayte Del C. Nerey; Sergio E. Pichuantes; Claudia P. Saavedra; Manuel A. Araya; Juan C. Tantaleán; Claudio C. Vásquez

doi:10.1007/s00284-001-0114-5

Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride

Mayte Del C. Nerey
, Sergio E. Pichuantes
, Claudia P. Saavedra
, Manuel A. Araya
, Juan C. Tantaleán
, Claudio C. Vásquez

Universidad de Santiago de Chile
Novartis

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cada or cadC/cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.

Original language	English
Pages (from-to)	187-190
Number of pages	4
Journal	Current Microbiology
Volume	45
Issue number	3
DOIs	https://doi.org/10.1007/s00284-001-0114-5
State	Published - 1 Sep 2002
Externally published	Yes

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title = "Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride",

abstract = "Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cada or cadC/cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.",

author = "Nerey, \{Mayte Del C.\} and Pichuantes, \{Sergio E.\} and Saavedra, \{Claudia P.\} and Araya, \{Manuel A.\} and Tantale{\'a}n, \{Juan C.\} and V{\'a}squez, \{Claudio C.\}",

year = "2002",

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doi = "10.1007/s00284-001-0114-5",

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T1 - Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride

AU - Nerey, Mayte Del C.

AU - Pichuantes, Sergio E.

AU - Saavedra, Claudia P.

AU - Araya, Manuel A.

AU - Tantaleán, Juan C.

AU - Vásquez, Claudio C.

PY - 2002/9/1

Y1 - 2002/9/1

N2 - Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cada or cadC/cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.

AB - Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cada or cadC/cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.

UR - https://www.scopus.com/pages/publications/0036717009

U2 - 10.1007/s00284-001-0114-5

DO - 10.1007/s00284-001-0114-5

M3 - Artículo

C2 - 12177740

AN - SCOPUS:0036717009

SN - 0343-8651

VL - 45

SP - 187

EP - 190

JO - Current Microbiology

JF - Current Microbiology

IS - 3

ER -

Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride

Abstract

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