TY - JOUR
T1 - Expression of Bacillus stearothermophilus LV cadmium resistance genes in Escherichia coli causes hypersensitivity to cadmium chloride
AU - Nerey, Mayte Del C.
AU - Pichuantes, Sergio E.
AU - Saavedra, Claudia P.
AU - Araya, Manuel A.
AU - Tantaleán, Juan C.
AU - Vásquez, Claudio C.
PY - 2002/9/1
Y1 - 2002/9/1
N2 - Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cada or cadC/cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.
AB - Determination of the nucleotide sequence of a 4.5-kb chromosomal DNA fragment of Bacillus stearothermophilus LV revealed two open reading frames (ORFs) of 121 and 727 amino acids (aa) that exhibit a high degree of similarity with the cadC and cadA cadmium resistance genes of a number of microorganisms. Transfer and expression of the B. stearothermophilus LV cada or cadC/cadA genes in E. coli caused increased cadmium chloride susceptibility in the bacterial host. Transfer of cadC alone did not result in any detectable phenotypic change in E. coli.
UR - http://www.scopus.com/inward/record.url?scp=0036717009&partnerID=8YFLogxK
U2 - 10.1007/s00284-001-0114-5
DO - 10.1007/s00284-001-0114-5
M3 - Artículo
C2 - 12177740
AN - SCOPUS:0036717009
SN - 0343-8651
VL - 45
SP - 187
EP - 190
JO - Current Microbiology
JF - Current Microbiology
IS - 3
ER -