Drug susceptibility testing and synergistic antibacterial activity of curcumin with antibiotics against enterotoxigenic Escherichia coli

Rangel Castañeda Itzia Azucena; Cruz Lozano José Roberto; Zermeño Ruiz Martin; Cortes Zarate Rafael; Hernández Hernández Leonardo; Tapia Pastrana Gabriela; Castillo Romero Araceli

doi:10.3390/antibiotics8020043

Drug susceptibility testing and synergistic antibacterial activity of curcumin with antibiotics against enterotoxigenic Escherichia coli

Rangel Castañeda Itzia Azucena, Cruz Lozano José Roberto, Zermeño Ruiz Martin, Cortes Zarate Rafael, Hernández Hernández Leonardo, Tapia Pastrana Gabriela, Castillo Romero Araceli

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Aim: This study investigated the susceptibility of Enterotoxigenic Escherichia coli to curcumin, as well as its synergistic effect with 12 antimicrobial drugs.Methods and Results: Our study shows that curcumin did not affect bacterial growth. The antimicrobial susceptibility of curcumin and antibiotic synergy were identified using disc diffusion on Mueller-Hinton agar. The strain of Enterotoxigenic Escherichia coli used was resistant to Ampicillin, Amoxicillin/Clavulanic acid, Ampicillin/Sulbactam, Ciprofloxacin, and Cefazolin. There was synergy between curcumin and the majority of antibiotics tested. Maximum synergy was observed with combinations of 330 μg/mL curcumin and Ceftazidime, followed by Cefotaxime, Amoxicillin/Clavulanic acid, Ampicillin, Aztreonam, Trimethoprim, Ciprofloxacin, Ceftriaxone, Cefazolin, Tetracycline, and Imipenem. Conclusion: Our findings indicated that curcumin might be useful as a combinatorial strategy to combat the antibiotic resistance of Enterotoxigenic Escherichia coli.

Original language	English
Article number	43
Journal	Antibiotics
Volume	8
Issue number	2
DOIs	https://doi.org/10.3390/antibiotics8020043
State	Published - Jun 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Antibacterial
Antibiotics
Curcumin
Escherichia coli
Synergism

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Drug susceptibility testing and synergistic antibacterial activity of curcumin with antibiotics against enterotoxigenic Escherichia coli",

abstract = "Aim: This study investigated the susceptibility of Enterotoxigenic Escherichia coli to curcumin, as well as its synergistic effect with 12 antimicrobial drugs.Methods and Results: Our study shows that curcumin did not affect bacterial growth. The antimicrobial susceptibility of curcumin and antibiotic synergy were identified using disc diffusion on Mueller-Hinton agar. The strain of Enterotoxigenic Escherichia coli used was resistant to Ampicillin, Amoxicillin/Clavulanic acid, Ampicillin/Sulbactam, Ciprofloxacin, and Cefazolin. There was synergy between curcumin and the majority of antibiotics tested. Maximum synergy was observed with combinations of 330 μg/mL curcumin and Ceftazidime, followed by Cefotaxime, Amoxicillin/Clavulanic acid, Ampicillin, Aztreonam, Trimethoprim, Ciprofloxacin, Ceftriaxone, Cefazolin, Tetracycline, and Imipenem. Conclusion: Our findings indicated that curcumin might be useful as a combinatorial strategy to combat the antibiotic resistance of Enterotoxigenic Escherichia coli.",

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author = "Azucena, {Rangel Casta{\~n}eda Itzia} and Roberto, {Cruz Lozano Jos{\'e}} and Martin, {Zerme{\~n}o Ruiz} and Rafael, {Cortes Zarate} and Leonardo, {Hern{\'a}ndez Hern{\'a}ndez} and Gabriela, {Tapia Pastrana} and Araceli, {Castillo Romero}",

note = "Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/antibiotics8020043",

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volume = "8",

journal = "Antibiotics",

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AU - Azucena, Rangel Castañeda Itzia

AU - Roberto, Cruz Lozano José

AU - Martin, Zermeño Ruiz

AU - Rafael, Cortes Zarate

AU - Leonardo, Hernández Hernández

AU - Gabriela, Tapia Pastrana

AU - Araceli, Castillo Romero

PY - 2019/6

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N2 - Aim: This study investigated the susceptibility of Enterotoxigenic Escherichia coli to curcumin, as well as its synergistic effect with 12 antimicrobial drugs.Methods and Results: Our study shows that curcumin did not affect bacterial growth. The antimicrobial susceptibility of curcumin and antibiotic synergy were identified using disc diffusion on Mueller-Hinton agar. The strain of Enterotoxigenic Escherichia coli used was resistant to Ampicillin, Amoxicillin/Clavulanic acid, Ampicillin/Sulbactam, Ciprofloxacin, and Cefazolin. There was synergy between curcumin and the majority of antibiotics tested. Maximum synergy was observed with combinations of 330 μg/mL curcumin and Ceftazidime, followed by Cefotaxime, Amoxicillin/Clavulanic acid, Ampicillin, Aztreonam, Trimethoprim, Ciprofloxacin, Ceftriaxone, Cefazolin, Tetracycline, and Imipenem. Conclusion: Our findings indicated that curcumin might be useful as a combinatorial strategy to combat the antibiotic resistance of Enterotoxigenic Escherichia coli.

AB - Aim: This study investigated the susceptibility of Enterotoxigenic Escherichia coli to curcumin, as well as its synergistic effect with 12 antimicrobial drugs.Methods and Results: Our study shows that curcumin did not affect bacterial growth. The antimicrobial susceptibility of curcumin and antibiotic synergy were identified using disc diffusion on Mueller-Hinton agar. The strain of Enterotoxigenic Escherichia coli used was resistant to Ampicillin, Amoxicillin/Clavulanic acid, Ampicillin/Sulbactam, Ciprofloxacin, and Cefazolin. There was synergy between curcumin and the majority of antibiotics tested. Maximum synergy was observed with combinations of 330 μg/mL curcumin and Ceftazidime, followed by Cefotaxime, Amoxicillin/Clavulanic acid, Ampicillin, Aztreonam, Trimethoprim, Ciprofloxacin, Ceftriaxone, Cefazolin, Tetracycline, and Imipenem. Conclusion: Our findings indicated that curcumin might be useful as a combinatorial strategy to combat the antibiotic resistance of Enterotoxigenic Escherichia coli.

KW - Antibacterial

KW - Antibiotics

KW - Curcumin

KW - Escherichia coli

KW - Synergism

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JF - Antibiotics

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ER -

Drug susceptibility testing and synergistic antibacterial activity of curcumin with antibiotics against enterotoxigenic Escherichia coli

Abstract

Bibliographical note

Keywords

UN SDGs

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