Anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb. in an experimental model of acute inflammation

Haydee Chávez; Angel T. Alvarado; Nesquen Tasayco-Yataco; Mario Pineda Pérez; Jorge A. García; María R. Bendezú; Felipe Surco-Laos; Juan J. Palomino-Jhong; Doris Laos-Anchante; Elizabeth J. Melgar-Merino; Nelly Vega-Ramos; Carmela Ferreyra-Paredes; Paulina Eliades Yarasca-Carlos; Dante Fermín Calderón-Huamaní; Mario Bolarte-Arteaga; Berta Loja-Herrera

doi:10.56499/jppres24.2100_13.2.444

Anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb. in an experimental model of acute inflammation

Haydee Chávez, Angel T. Alvarado, Nesquen Tasayco-Yataco, Mario Pineda Pérez, Jorge A. García, María R. Bendezú, Felipe Surco-Laos, Juan J. Palomino-Jhong, Doris Laos-Anchante, Elizabeth J. Melgar-Merino, Nelly Vega-Ramos, Carmela Ferreyra-Paredes, Paulina Eliades Yarasca-Carlos, Dante Fermín Calderón-Huamaní, Mario Bolarte-Arteaga, Berta Loja-Herrera

FARMACIA Y BIOQUIMICA

Research output: Contribution to journal › Article › peer-review

Abstract

Context: Acute inflammation due to persistent stimulation of noxes evolves into chronic inflammation that contributes to the pathogenesis of asthma, arthritis, atherosclerosis, diabetes, aging, and cancer; therefore, it is justified to study plants with anti-inflammatory activity and fewer adverse effects. Aims: To evaluate the potential anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb (HELSA) in an experimental model of acute inflammation induced by carrageenan in rats. Methods: The total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, total polyphenolic content (TPC) by the Folin-Ciocalteu method, and antioxidant activity by two in vitro methods. The oral median lethal dose (LD50) was determined by the logarithmic method, and acute inflammation was induced by 1% carrageenan in rats. Results: The TFC was 1.63 ± 0.02 mg QE/100 mg and TPC was 22.90 ± 0.05 mg GAE/100 mg. Antioxidant capacity: DPPH IC50 2.89 ± 0.04 µg/µL; ABTS 11.67 ± 0.03 mM TEAC/100 mg dry extract. Correlation relationship TFC/DPPH (r = -0.9941; R² = 0.9884), TFC/ABTS (r = 0.9971; R² = 0.9944), TPC/DPPH (r = 0.5694; R² = 0.3243) and TPC/ABTS (r = -0.5960; R² = 0.3553). The LD50 was 9602 mg/kg. A significant decrease in rat paw edema (p<0.05) was evident from the first hour, in contrast to the negative control. The highest percentage of anti-inflammatory inhibition (75.75%) was at 7 hours with a dose of 500 mg/kg of HELSA, being higher than the effect of ibuprofen (39.40%) and lower than dexamethasone (87.87%). Conclusions: The total flavonoid content and polyphenols of the hydroalcoholic extract of the leaves of S. alata exhibit potential anti-inflammatory activity in a model of acute inflammation that should be examined with other experimental models to initiate clinical trials in humans.

Translated title of the contribution	Efecto anti-inflamatorio del contenido total de flavonoides del extracto hidroalcohólico de las hojas de Senna alata (L.) Roxb. en un modelo experimental de inflamación aguda
Original language	English
Pages (from-to)	444-458
Number of pages	15
Journal	Journal of Pharmacy and Pharmacognosy Research
Volume	13
Issue number	2
DOIs	https://doi.org/10.56499/jppres24.2100_13.2.444
State	Published - Mar 2025

Bibliographical note

Publisher Copyright:
© 2025 Journal of Pharmacy & Pharmacognosy Research.

Keywords

anti-inflammatory effect
antioxidant activity in vitro
biophenols
flavonoids
Senna alata

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.56499/jppres24.2100_13.2.444

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Chávez, H., Alvarado, A. T., Tasayco-Yataco, N., Pérez, M. P., García, J. A., Bendezú, M. R., Surco-Laos, F., Palomino-Jhong, J. J., Laos-Anchante, D., Melgar-Merino, E. J., Vega-Ramos, N., Ferreyra-Paredes, C., Yarasca-Carlos, P. E., Calderón-Huamaní, D. F., Bolarte-Arteaga, M., & Loja-Herrera, B. (2025). Anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb. in an experimental model of acute inflammation. Journal of Pharmacy and Pharmacognosy Research, 13(2), 444-458. https://doi.org/10.56499/jppres24.2100_13.2.444

@article{3dd5e698057e4d1f9a85941ad6979a89,

title = "Anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb. in an experimental model of acute inflammation",

abstract = "Context: Acute inflammation due to persistent stimulation of noxes evolves into chronic inflammation that contributes to the pathogenesis of asthma, arthritis, atherosclerosis, diabetes, aging, and cancer; therefore, it is justified to study plants with anti-inflammatory activity and fewer adverse effects. Aims: To evaluate the potential anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb (HELSA) in an experimental model of acute inflammation induced by carrageenan in rats. Methods: The total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, total polyphenolic content (TPC) by the Folin-Ciocalteu method, and antioxidant activity by two in vitro methods. The oral median lethal dose (LD50) was determined by the logarithmic method, and acute inflammation was induced by 1% carrageenan in rats. Results: The TFC was 1.63 ± 0.02 mg QE/100 mg and TPC was 22.90 ± 0.05 mg GAE/100 mg. Antioxidant capacity: DPPH IC50 2.89 ± 0.04 µg/µL; ABTS 11.67 ± 0.03 mM TEAC/100 mg dry extract. Correlation relationship TFC/DPPH (r = -0.9941; R2 = 0.9884), TFC/ABTS (r = 0.9971; R2 = 0.9944), TPC/DPPH (r = 0.5694; R2 = 0.3243) and TPC/ABTS (r = -0.5960; R2 = 0.3553). The LD50 was 9602 mg/kg. A significant decrease in rat paw edema (p<0.05) was evident from the first hour, in contrast to the negative control. The highest percentage of anti-inflammatory inhibition (75.75%) was at 7 hours with a dose of 500 mg/kg of HELSA, being higher than the effect of ibuprofen (39.40%) and lower than dexamethasone (87.87%). Conclusions: The total flavonoid content and polyphenols of the hydroalcoholic extract of the leaves of S. alata exhibit potential anti-inflammatory activity in a model of acute inflammation that should be examined with other experimental models to initiate clinical trials in humans.",

keywords = "anti-inflammatory effect, antioxidant activity in vitro, biophenols, flavonoids, Senna alata",

author = "Haydee Ch{\'a}vez and Alvarado, {Angel T.} and Nesquen Tasayco-Yataco and P{\'e}rez, {Mario Pineda} and Garc{\'i}a, {Jorge A.} and Bendez{\'u}, {Mar{\'i}a R.} and Felipe Surco-Laos and Palomino-Jhong, {Juan J.} and Doris Laos-Anchante and Melgar-Merino, {Elizabeth J.} and Nelly Vega-Ramos and Carmela Ferreyra-Paredes and Yarasca-Carlos, {Paulina Eliades} and Calder{\'o}n-Huaman{\'i}, {Dante Ferm{\'i}n} and Mario Bolarte-Arteaga and Berta Loja-Herrera",

note = "Publisher Copyright: {\textcopyright} 2025 Journal of Pharmacy & Pharmacognosy Research.",

year = "2025",

month = mar,

doi = "10.56499/jppres24.2100_13.2.444",

language = "Ingl{\'e}s",

volume = "13",

pages = "444--458",

journal = "Journal of Pharmacy and Pharmacognosy Research",

issn = "0719-4250",

publisher = "Asociacion Academica de Ciencias Farmaceuticas de Antofagasta (ASOCIFA)",

number = "2",

}

Chávez, H, Alvarado, AT, Tasayco-Yataco, N, Pérez, MP, García, JA, Bendezú, MR, Surco-Laos, F , Palomino-Jhong, JJ, Laos-Anchante, D, Melgar-Merino, EJ, Vega-Ramos, N, Ferreyra-Paredes, C, Yarasca-Carlos, PE, Calderón-Huamaní, DF, Bolarte-Arteaga, M & Loja-Herrera, B 2025, 'Anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb. in an experimental model of acute inflammation', Journal of Pharmacy and Pharmacognosy Research, vol. 13, no. 2, pp. 444-458. https://doi.org/10.56499/jppres24.2100_13.2.444

Anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb. in an experimental model of acute inflammation. / Chávez, Haydee; Alvarado, Angel T.; Tasayco-Yataco, Nesquen et al.
In: Journal of Pharmacy and Pharmacognosy Research, Vol. 13, No. 2, 03.2025, p. 444-458.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb. in an experimental model of acute inflammation

AU - Chávez, Haydee

AU - Alvarado, Angel T.

AU - Tasayco-Yataco, Nesquen

AU - Pérez, Mario Pineda

AU - García, Jorge A.

AU - Bendezú, María R.

AU - Surco-Laos, Felipe

AU - Palomino-Jhong, Juan J.

AU - Laos-Anchante, Doris

AU - Melgar-Merino, Elizabeth J.

AU - Vega-Ramos, Nelly

AU - Ferreyra-Paredes, Carmela

AU - Yarasca-Carlos, Paulina Eliades

AU - Calderón-Huamaní, Dante Fermín

AU - Bolarte-Arteaga, Mario

AU - Loja-Herrera, Berta

PY - 2025/3

Y1 - 2025/3

N2 - Context: Acute inflammation due to persistent stimulation of noxes evolves into chronic inflammation that contributes to the pathogenesis of asthma, arthritis, atherosclerosis, diabetes, aging, and cancer; therefore, it is justified to study plants with anti-inflammatory activity and fewer adverse effects. Aims: To evaluate the potential anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb (HELSA) in an experimental model of acute inflammation induced by carrageenan in rats. Methods: The total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, total polyphenolic content (TPC) by the Folin-Ciocalteu method, and antioxidant activity by two in vitro methods. The oral median lethal dose (LD50) was determined by the logarithmic method, and acute inflammation was induced by 1% carrageenan in rats. Results: The TFC was 1.63 ± 0.02 mg QE/100 mg and TPC was 22.90 ± 0.05 mg GAE/100 mg. Antioxidant capacity: DPPH IC50 2.89 ± 0.04 µg/µL; ABTS 11.67 ± 0.03 mM TEAC/100 mg dry extract. Correlation relationship TFC/DPPH (r = -0.9941; R2 = 0.9884), TFC/ABTS (r = 0.9971; R2 = 0.9944), TPC/DPPH (r = 0.5694; R2 = 0.3243) and TPC/ABTS (r = -0.5960; R2 = 0.3553). The LD50 was 9602 mg/kg. A significant decrease in rat paw edema (p<0.05) was evident from the first hour, in contrast to the negative control. The highest percentage of anti-inflammatory inhibition (75.75%) was at 7 hours with a dose of 500 mg/kg of HELSA, being higher than the effect of ibuprofen (39.40%) and lower than dexamethasone (87.87%). Conclusions: The total flavonoid content and polyphenols of the hydroalcoholic extract of the leaves of S. alata exhibit potential anti-inflammatory activity in a model of acute inflammation that should be examined with other experimental models to initiate clinical trials in humans.

AB - Context: Acute inflammation due to persistent stimulation of noxes evolves into chronic inflammation that contributes to the pathogenesis of asthma, arthritis, atherosclerosis, diabetes, aging, and cancer; therefore, it is justified to study plants with anti-inflammatory activity and fewer adverse effects. Aims: To evaluate the potential anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb (HELSA) in an experimental model of acute inflammation induced by carrageenan in rats. Methods: The total flavonoid content (TFC) was determined by spectrophotometry at 510 nm, total polyphenolic content (TPC) by the Folin-Ciocalteu method, and antioxidant activity by two in vitro methods. The oral median lethal dose (LD50) was determined by the logarithmic method, and acute inflammation was induced by 1% carrageenan in rats. Results: The TFC was 1.63 ± 0.02 mg QE/100 mg and TPC was 22.90 ± 0.05 mg GAE/100 mg. Antioxidant capacity: DPPH IC50 2.89 ± 0.04 µg/µL; ABTS 11.67 ± 0.03 mM TEAC/100 mg dry extract. Correlation relationship TFC/DPPH (r = -0.9941; R2 = 0.9884), TFC/ABTS (r = 0.9971; R2 = 0.9944), TPC/DPPH (r = 0.5694; R2 = 0.3243) and TPC/ABTS (r = -0.5960; R2 = 0.3553). The LD50 was 9602 mg/kg. A significant decrease in rat paw edema (p<0.05) was evident from the first hour, in contrast to the negative control. The highest percentage of anti-inflammatory inhibition (75.75%) was at 7 hours with a dose of 500 mg/kg of HELSA, being higher than the effect of ibuprofen (39.40%) and lower than dexamethasone (87.87%). Conclusions: The total flavonoid content and polyphenols of the hydroalcoholic extract of the leaves of S. alata exhibit potential anti-inflammatory activity in a model of acute inflammation that should be examined with other experimental models to initiate clinical trials in humans.

KW - anti-inflammatory effect

KW - antioxidant activity in vitro

KW - biophenols

KW - flavonoids

KW - Senna alata

UR - http://www.scopus.com/inward/record.url?scp=85211730162&partnerID=8YFLogxK

U2 - 10.56499/jppres24.2100_13.2.444

DO - 10.56499/jppres24.2100_13.2.444

M3 - Artículo

AN - SCOPUS:85211730162

SN - 0719-4250

VL - 13

SP - 444

EP - 458

JO - Journal of Pharmacy and Pharmacognosy Research

JF - Journal of Pharmacy and Pharmacognosy Research

IS - 2

ER -

Anti-inflammatory effect of the total flavonoid content of the hydroalcoholic extract of the leaves of Senna alata (L.) Roxb. in an experimental model of acute inflammation

Abstract

Bibliographical note

Keywords

UN SDGs

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