AISLAMIENTO, IDENTIFICACIÓN Y EVALUACIÓN IN VITRO DE AISLADOS NATIVOS DE Bacillus, Trichoderma Y Streptomyces CON POTENCIAL PARA EL BIOCONTROL DE LOS HONGOS DE LA MADERA DE LA VID

Translated title of the contribution: ISOLATION, IDENTIFICATION AND IN VITRO EVALUATION OF NATIVE ISOLATES OF Bacillus, Trichoderma AND Streptomyces WITH POTENTIAL FOR THE BIOCONTROL OF GRAPEVINE TRUNK FUNGI

Arturo Morales-Pizarro, Javier Javier-Alva, L. A. Álvarez, Rocío Mayta-Obos, René Aguilar-Anccota, Ricardo Peña-Castillo, Roger Chanduvi-García, David Lindo-Seminario

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Abstract

Background: Biocontrol of grapevine trunk diseases (GTDs) is a friendly alternative to chemical control that cause resistance and contamination of the ecosystems. Objective: isolate native grapevine root and rhizosphere microorganisms with biocontrol potential of grapevine trunk fungi diseases. Methodology: The competition for substrate (CS) was studied with the Bell's scale, contact time (CT) between the antagonist-pathogen (days) and the growth inhibition percentage (GIP). Results: Seven Trichoderma strains were isolated (from roots: Tr-1, Tr-2, Tr-3, Tr-6; and from rhizosphere: Tr-4, Tr-5 Tr-7); six Bacillus spp. (from roots: Bac-4, Bac-5, Bac-6; and from rhizosphere: Bac-1, Bac-2, Bac-3); and two Streptomyces sp. (Act-1, Act-2). The CS results evaluated with the Bell scale showed Tr-1, Tr-7, T.atv (Trichoderma atroviride) and T-22 (Trichoderma harzianum) were placed in class 1 against Lasiodiplodia theobromae and, all isolates were placed in class 1 against Campylocarpon pseudofasciculare and Phaeoacremonium parasiticum. The CT of Trichoderma was 2 days (L. theobromae) and 3 days (C. pseudofasciculare and P. parasiticum). In the antibiosis test, Tr-5 (Trichoderma) and Bac-3 (Bacillus) had the highest GIP values (>50%) against the different evaluated pathogens. The isolate Act-2 (Streptomyces sp.) presented a GIP, >70% (L. theobromae), and >40% (C. pseudofasciculare) and >30% (P. parasiticum). Implications: The use of biological control in the management of grapevine trunk diseases is an effective tool, and can be integrated into an integrated management strategy for these pathologies. Conclusions: The native isolates Bac-3 (Bacillus spp.), Tr-5 (Trichoderma spp.), Act-2 (Streptomyces sp.) demonstrated in vitro control against the pathogens L. theobromae, C. pseudofasciculare and P. parasiticum, pathogens associated to grapevine trunk diseases.

Translated title of the contributionISOLATION, IDENTIFICATION AND IN VITRO EVALUATION OF NATIVE ISOLATES OF Bacillus, Trichoderma AND Streptomyces WITH POTENTIAL FOR THE BIOCONTROL OF GRAPEVINE TRUNK FUNGI
Original languageSpanish
Article number4206
JournalTropical and Subtropical Agroecosystems
Volume25
Issue number2
DOIs
StatePublished - May 2022
Externally publishedYes

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