A simple and efficient method for highquality total RNA isolation from oleaginous microalgae

Juan C. Castro, Hicler N. Rodríguez, J. Dylan Maddox, Bruce Jiu, Joe B. Petterman, Jorge L. Marapara, Marianela Cobos

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Microalgae are a very diverse group of microorganisms with great potential for biotechnological applications. To best exploit these organisms is necessary to expand our knowledge at its molecular levels. To realize these studies, however, it is essential to have efficient and reproducible methods for total RNA isolation. The objective of this research was to develop a method for high-quality total RNA isolation from three oleaginous microalgae species promissories for biotechnological applications. Three strains Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp. were cultured on CHU-10 medium. Then, were harvested by centrifugation and total RNA was isolated with three commercial kits, one previously published method, and our improved CTAB-LiCl method. Quality and quantity of total RNA isolated were assayed with standard spectrophotometric and electrophoretic techniques. The highest quality (A260/A280: 1.95-2.02, A260/A230: 1.12-1.84) and yield (16.40-93.63 μg/g fresh weight) of total RNA from the three microalgae strains were obtained with our improved CTAB-LiCl method. In addition, the time required to complete the isolation process and the cost were relatively lowest. In conclusion, the improved CTAB-LiCl method, in contrast to frequently used commercial kits, and a previously published method, is effective and reliable to produce total RNA of high quality and quantity that is suitable for cDNA synthesis and polymerase chain reaction.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalPlant Cell Biotechnology and Molecular Biology
Volume18
Issue number1-2
StatePublished - 2017
Externally publishedYes

Bibliographical note

Funding Information:
This research was supported by the Programa Nacional de Innovación para la Competitividad y Productividad (PNICP) with grant contract No. 383-PNICP-PIBA-2014.

Publisher Copyright:
© Copyright International Knowledge Press. All rights reserved.

Keywords

  • Ankistrodesmus
  • Chlorella
  • Oleaginous microalgae
  • RNA isolation
  • Scenedesmus

Fingerprint

Dive into the research topics of 'A simple and efficient method for highquality total RNA isolation from oleaginous microalgae'. Together they form a unique fingerprint.

Cite this